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S7804 Fiches technique(PDF) 21 Page - List of Unclassifed Manufacturers

No de pièce S7804
Description  CpG WIZ Prader-Willi/Angelman Amplification Kit
PDF  32 Pages
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Fabricant  ETC [List of Unclassifed Manufacturers]
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S7804 Fiches technique(HTML) 21 Page - List of Unclassifed Manufacturers

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17
STEP 3. Gel Electrophoresis
a. After the completion of PCR, add an appropriate amount of loading dye to
the sample and analyze 5 µL of the reaction on a 2% agarose or a 10% native
acrylamide or other high resolution agarose gel. Use DNA markers (100-300
bp range) to determine the size of PCR products.
b. After electrophoresis, stain the gel with ethidium bromide. Dilute the 10
mg/mL stock solution 1:10,000 in deionized water. Stain for 10-30 minutes
and destain for 10-30 minutes in deionized water at room temperature.
Note: Ethidium bromide is a known carcinogen. Exercise appropriate
caution and good lab practice when using this reagent.



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